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Zeiss Axio-Imager Z2 upright microscope

J-A Bombardier Building, Room 3223-01
Advanced Microscope Tier 1 usage price

Instrument awarded to Dr. Pascal Chartrand by the Canadian Foundation for Innovation (CFI)

  • Applications
    • Transmitted light
    • Interference Contrast (DIC)
    • Fluorescence

  • Light sources

    • 12V 100W halogen lamp for transmitted light

    • 200W Exact X-Cite Lamp (340~675nm) for Fluorescence

Full specifications of the exact X-Cite lamp

Peak emission (nm)Power (mW)
3345
36520
40519
43622
54621
57918

X-Cite Exact Quick Start Guide (English pdf)
X-Cite Exact User Guide (English pdf)
Comparison between HBO vs X-Cite Exact Mercury Lamp Emission Spectra (English) ( Source )

  • Objectives

  • 10x/0.30 Air WD 5.30

  • 20x/0.80 Air WD 0.61

  • 40x/0.75 Air WD 0.71

  • 63x/1.40 WD Oil 0.19

  • 100x/1.30  WD 0.20 oil

  • 100x/1.40  WD 0.17 oil

  • Full lens specifications

BF: Bright-field
DIC: Interference contrast

Filter cubes

  1. DAPI
  2. GFP
  3. YFP
  4. DsRed/Cy3
  5. TxRed
  6. Cy3.0
  7. Cy3.5
  8. Cy5
  9. DIC analyzer

  10. Empty


Complete filter specifications

PositionNameBrandIdentifierExcitation filter Dichroic mirrorEmission filterComment

1DAPI
Filter Set 49		Zeiss

488049-9901-000

365/50
[ 340-390]		395LP445/50
[420 -470]


2GFP
Filter Set 38		Zeiss000000-1031-346

470/40
[450 -490]

495LP

525/50
[500 -550]
FT 495BP 525/50
3YFP
Filter Set 46		Zeiss

000000-1196-681

500/20
[490 -510]		515LP535/30
[520 -550]


4DsRed
Filter Set 43		Zeiss

000000-1114-101

545/25
[533 -557]		570LP605/70
[570 -640]		FT570BP 605/70
5TxRed
Filter Set 45		Zeiss000000-1114-462560/40
[540 -580]		585LP630/75
[593 -667]


6Cy3.0

Chroma

SP102v1

546/11
[541 -551]

557LP567/15
[560 -574]


7Cy3.5

ChromaSP103v1

581/10
[576 -586]

593LP617/40
[597 -637]


8Cy5 narrowChroma49009

640/30
[625 -655]

660LP690/50
[665 -715]


9DIC analyzerZeiss






10Empty








Detector

Startup
  1. Remove the protective cover from the microscope
  2. Turn on the computer ( #1 )

  3. Turn on the exact X-Cite lamp on the shelf to the left of the microscope ( #2)

    The exact X-Cite lamp display will flash during heating (approximately 4 minutes) and will then be operational.

    The exact X-Cite lamp must be on  for at least 30 minutes  before being turned off  and vice versa

  4. Turn on the power bar on the shelf above the computer ( #3)

  5. Press the microscope start button located on the rear left (#4)

  6. Use your UdM credentials to log in to Windows

    When using it for the first time, it is necessary to import the microscope parameters into the software. To do this, follow the First Use instructions .

  7. Start the Zen Blue software







The following diagrams allow you to follow the light path in transmitted light (bright field, DIC) and in reflected light (fluorescence).


  • Objective 10-0.3 Plan NeoFlura #420340-9901-000 added
  • Parafocality adjustment using 100x-1.4 as reference
  • Condenser removed and cleaned (broken glass)
  • Zen 2.6 updated hotfix 12
  • Microsoft Windows updated
  • X-Cite Liquid light guide replaced (Transmittance was 75% remaining)
  • Fluorescence Light bulb replaced (was 2593 hours)
  • Power output at the sample using 20x objective GFP cube 488nm is 116mW
  • Objective parafocality adjusted
  • Objective Focus speed was changed 10x: 7; 20x: 6; 4-x: 5; 63x: 3; 100x-1.3: 3; 100x-1.4: 3.
  • Condenser lens cleaned (oil)
  • Data storage OK
  • Added to wiki

Stand

  • Zeiss AxioImager Z2 upright Serial: 35230001000
    Part Number: 430000-9902
    System ID 1022265893

  • Camera adapter Model 60N-C, 1", 1x, Model: 426114

  • Motorized Neutral density filters for transmitted light

  • Manual Field diaphragm for transmitted light

  • Manual polarizer
  • Left imaging port with manual splitter camera adapter Model 60N-C, 1", 1x, Model: 426114
  • Trinocular with 100% ocular 40% occular/70% camera and 100% manual splitter
  • 3mm liquid light guide #805-0038
  • Zeiss 423302-0000 Collimator
  • Motorized Aperture diaphragm
  • Motorized Fluorescence field diaphragm

Light sources

  • Transmitted Halogen light 12V 100W HAL 100 #423000
    • TBD Filters
  • X-Cite exacte 200 W Model XCT10A Serial: XCT10A-0156

Condenser

  • Motorized condenser #424201-9902

  • Lens NA 0.9 WD TBD Part Number: TBD

  • Manual polarizer

  • Filter turret 6 positions manual

    1. H Empty

    2. D Darkfield
    3. DIC III #426706
    4. Ph3
    5. DICII #426702

    6. Ph 2

    7. DIC 426701
    8. Ph 1

Objectives

  1. 10x/0.30 Air WD 5.30 DIC I  Plan-NeoFluar M27 420340-9901-000

  2. 20x/0.80 Air WD 0.61 DIC II Plan-Apochromat W0.8x1/36" 440640-9903-000

  3. 40x/0.75 Air WD 0.71 DIC II EC Plan-NeoFluar M27 420360-9900-000

  4. 63x/1.40 Oil WD 0.19 DIC III Plan-Apochromat M27 420782-9900-000

  5. 100x/1.30 Oil WD 0.20 DIC III Plan-NeoFluar M27 420490-9900-000

  6. 100x/1.40 Oil WD 0.17 DIC III Plan-Apochromat M27 420792-9900-000 

Stage

  • Motorized stage Zeiss AIM System #2502000124
  • Remote control joystick
  • Inserts
    • Slide only

Filters

10-positions motorized filter wheel #424913 #424905-0160-810

  1. DAPI Zeiss Filter Set 49 cube 424933
  2. GFP Zeiss  Filter Set 38 cube 424933
  3. YFP Zeiss  Filter Set 46 cube 424933
  4. DsRed Zeiss  Filter Set 43 cube 424933
  5. TxRed Zeiss  Filter Set 45 cube 424933
  6. Cy3.0 Chroma #SP102v1 C162410
  7. Cy3.5 Chroma #SP103v1 C162411
  8. Cy5 Chroma #49009 C162412
  9. DIC Analyzer Zeiss 424932-01
  10. Empty

Detector

  • Photometrics Prime Camera sCMOS 2048 x 2048 pixels, 16-bit, 30 images/s at full resolution, detector size 13.312mm x 13.312mm (18.8 mm diagonal). Serial: A18B202001. Cameralink cable and PCIe adapter available for imaging 100 images/s

Workstation

  • HP Z800 Workstation Serial: CZC1473Y0Q Part number: WJ112ECJ#AK6
  • 2 x Intel Xeon X5650 2.66 GHz
  • RAM 24 GB DDR3 1333 MHz ECC (12 x 2 GB)
  • OS 500 GB SSD 410 MB/s
  • 2 TB HD Data Storage (2 x 1 TB spanned volume) 110 MB/s
  • Video Card ATI FirePro V5800 1 GB DDR5
  • Monitor Dell ST2410  24' 1920 x 1080
  • Software Zen Blue 2.6 Hotfix 12

Incubation

  • None

Consumables


Can I use this microscope to look at cell in a dish?

  • No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.


Core Facilities CIB               Structural Biology    |    Cytometry    |    Microscopy    |    Histology

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