Nikon Eclipse E-600 upright microscope

Roger Gaudry N-620

• Applications
  • Brightfield
  • Phase Contrast
  • Polarized light
  • Fluorescence

• Light sources

  • Halogen lamp for transmitted light

  • Mercury lamp (350~600nm) for fluorescence 

• Objectives:

  • 10x/0.3 Air Ph1 WD 16.0 (Transmission curve)

  • 40x/0.75 Air Ph2 WD 0.72 (Transmission curve)

  • 100x/1.3 Oil Ph3 WD 0.2 (Transmission curve) 

• Filter cubes (Specifications)

  • DAPI/Hoechst/AMCA
  • FITC/EGFP

  • TRITC/Rhodamine

  • TxRed

• Detector

  • Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6fps at full frame

Startup

1. Turn on the computer
2. Turn on the microscope power bar

3. If fluorescence is required, turn on the mercury lamp power supply unit (3A) and press ignition (3B)

   Mercury lamp must be on for at least 30 min before being turned off and vice-versa

4. Log in Windows using your UdM credentials

5. Start-up NIS-Elements

   The first time you log in the computer, you need to import the microscope settings. To do this follow the instructions Nikon Eclipse E600-en

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Shutdown

1. Save your data
2. Close NIS-Elements
3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
4. Get your samples from the microscope
5. If used, clean the oil objective with lens cleaner and lens paper
6. If fluorescence was used, turn off the mercury lamp power supply unit (3A)
7. Turn off the microscope power bar (2)
8. Wait until the lamps are cool and cover the microscope

Storage Management

• Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
• At the end of each session, copy your data to your external drive and delete it from the local C: drive
• You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
• In any case, your files should be removed from the C: drive.

Setting-up NIS-Elements

This is required the first time you are using the instrument. You will usually do it during the training session. It can be performed if something is not working properly right or if you want to refresh the software interface.

1. Close NIS-Elements
2. Wait until the complete closure of NIS-Elements
3. Open the folder Desktop\Logiciels
4. Open the software NIS Settings Utility
5. Click on the Import tab
6. Click on Browse
7. Navigate to your desktop
8. Select the file Nikon-E600 Settings.bin
9. Click Select
10. Select all items
11. Click Import
12. Click OK
13. Close the NIS Settings

Follow the schematics below for Transmitted light (Bright field, Phase Contrast, Polarized light) and transmitted light (fluorescence).

• Nikon_Eclipse-E600_Manual.pdf
• Nikon_Eclipse-E600_Polarization_Manual.pdf
• Nikon_Eclipse-E600_Epi Fluo D_Manual.pdf
• Nikon_Eclipse-E600_Epi Fluo Y_Manual.pdf
• Nikon_Camera DS-Ri2_Manual.pdf
• NIS-Elements_BR_Manual.pdf

To do

• Adjust focus drive jitter
• Add a holder for polarized light analyzer

2021-07-20

• Replacement mercury lamp bulb HBO 1003W/2
• 256 GB SSD added in workstation for OS
• Windows 10 Installation
• BIOS updated to v2.47
• Camera Firmware updated to v2.11
• NIS-Elements Basic Research v4.6 64-bits installed
• Creation NIS Elements Settings
• FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

2018-04-04

• Nikon Preventive Maintenance
• Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
• 100x slightly damaged but not the lens
• Fluorescence cubes damaged: FITC TxRed
• Focus drive has a jutter

Stand

• Nikon Eclipse E600 upright Serial 725540

Light sources

• Transmitted light Halogen 12V 100W Serial 01875599

• Reflected light

  • Power supply Nikon Mercury lamp Type C SHG1 Serial D12139

  • Bulb housing model LH M100C-1 Serial 039326

Condenser

• Condenser Universal C-CU Serial 081901
• Lens Dry NA 0.9
• Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

• 10x/0.3 Plan Fluor Ph1 DLL WD 16.0 Air (Transmission curve)

• 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air (Transmission curve) with PF40 Wollaston prism

• 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil (Transmission curve) with PF/PA 100 Oil Wollaston prism

• Empty
• Empty
• Empty

Turret

Detector

• Camera Nikon DS-Ri2 Serial 701234

Workstation

• HP Z440 Workstation
  • Intel Xeon E5-1630 v3 @ 3.7GHz
  • RAM 32 GB DDR4 2133 MHz  (4 x 8 GB)
  • OS 256GB SSD 550 MBs
  • 2TB HD Data Storage 150 MBs
  • Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
• Monitor HP Z24i display 24' 1920x1200

Consumables

• Mercury lamp bulb OSRAM HBO 1003W/2 100W

• Tungsten Halogen Lamp OSRAM 64623 HLX 100W 12V