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Leica DM2000 Upright Microscope
Desmarais Building, Room 3248
Available upon request to Dr Samaha Lab
- Applications
Bright-field
- Fluorescence
- Color camera
Light sources
Halogen lamp 30 W for transmitted light
Mercury lamp 100 W (350~600nm) for fluorescence
Peak Wavelength (nm) | Power (mW) |
---|---|
334 | 7 |
365 | 45 |
405 | 34 |
436 | 43 |
546 | 37 |
579 | 26 |
HBO Mercury lamp emission spectra (Source)
Comparison HBO Mercury vs XCite Metal Halide Lamps (Source)
OSRAM HBO 100W/2 Mercury lamp manual (pdf)
Objectives
10x/0.3 Air WD 11
20x/0.5 Air WD 1.15
40x/0.75 Air WD 0.37
Position | Name | Brand | Full name | Product ID | Magnification | Numerical aperture | Immersion | Type | Working distance (mm) | Transmittance (% [nm]) | Technique | Coverglass thickness (mm) |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 10x/0.3 Air | Leica | 10x/0.3 Air HC Plan Fluotar | 5006505 | 10x | 0.3 | Air | Plan Fluor | 11 | >80% [385-775] | BF, Fluo | 0.17 |
2 | 20x/0.5 Air | Leica | 20x/0.5 Air HC Plan Fluotar | 506503 | 20x | 0.5 | Air | Plan Fluor | 1.15 | >80% [395-800] | BF, Fluo | 0.17 |
3 | 40x/0.75 | Leica | 40x/0.75 Air HC Plan Fluotar | 506144 | 40x | 0.75 | Air | Plan Fluor | 0.37 | >80% [420-850] | BF, Fluo | 0.17 |
4 | Empty | |||||||||||
5 | Empty | |||||||||||
6 | Empty |
- Filter cubes
- I3 (GFP, YFP)
- N2.1 (TRITC)
Position | Name | Brand | ID | Excitation filter | Dichroic mirror | Emission filter | Comments |
---|---|---|---|---|---|---|---|
1 | I3 | Leica | I3 | 470/40 [450-490] | 510LP | 515LP [520+] | Emission filter is very broad. Be aware of bleed-through. |
2 | N2.1 | Leica | N2.1 | 537/45 | 580LP | 590LP [595+] | Emission filter is very broad. Be aware of bleed-through. |
3 | Empty | ||||||
4 | Empty | ||||||
5 | Empty |
- Detector
- Color camera Leica DFC425 C 2592 × 1944 pixels, 14-bit in monochrome mode , 36-bits in color mode, 6 frame/s
Serial 535111411
- Color camera Leica DFC425 C 2592 × 1944 pixels, 14-bit in monochrome mode , 36-bits in color mode, 6 frame/s
- Turn on the computer (#1)
If fluorescence is required, turn on the mercury lamp power supply unit (#2)
Mercury lamp must be on for at least 30 min before being turned off and vice-versa
- If transmitted light is required, turn on the halogen lamp on the right side of the microscope (#3)
- Log in Windows using your UdM credentials
- Start-up LAS
The first time you use LAS, you will be asked for registration and activation.
- Select Do not tell me about this again at the bottom left
- Click Close
- Save your data
- Close LAS
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Turn off the computer
- If fluorescence was used, turn off the mercury lamp power supply unit (#2)
- If transmitted light was used, turn off the halogen lamp on the right side of the microscope (#3)
Wait until the lamps are cool and cover the microscope
Important Reminders
- Take back your samples including ones in the microscope
- Leave the microscope and the working area clean
- Mercury lamp must be on for at least 30 min before being turned off and vice-versa
- Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
The first time you use LAS, you will be asked for registration and activation.
- Select Do not tell me about this again at the bottom left
- Click Close
The following schematics depict the lightpaths for transmitted (bright-field and phase contrat) and reflected (fluorescence) lights.
Available manuals
- Bring test slides
- Added to Wiki
- Computer replacement
- Windows 10 installation
- Network connection
Stand
- Leica DM2000 upright Serial 3312222-022011
Light sources
- Transmitted Halogen 30W
- Reflected light
Power supply Ebq 100-04-L Serial 11 1111 0103B00931
Leica Mercury lamp housing Product ID 11504114
Condenser
- TBD
Condenser filters
- DF Filters 501158 (looks like phase ring)
Objectives
- Nikon Plan Fluor 4x/0.13 MRH000041 (should not be used)
Leica HCX PL Fluotar 10x/0.3 506505
Leica HCX PL Fluotar20x/0.5 506503
Leica HCX PL Fluotar40x/0.75 506144
Stage
- Manual Stage TBD
Filters
- Leica I3
- Leica N2.1
- Empty
- Empty
- Empty
- Empty
Cube Leica 11513882
Detector
- Leica DFC425C Serial 535111411 Color Camera 2592 × 1944 pixels, 14-bit monochrome, 36-bit color mode, 6 image/s at full resolution
Workstation
- HP Z440 Workstation
- Intel Xeon E5-1620 v4 @ 3.5GHz
- RAM 32 GB DDR4 2400 MHz ECC (4 x 8 GB)
- OS 500GB SSD 550 MB/s
- 4TB HD Data Storage (2 x 2 TB spanned volume) 170 MB/s
- Video Card nVidia GTX 1080 8GB DDR5 dedicated memory
- Monitor HP Z24i display 24' 1920x1200
- Software NIS-Elements AR v5.02
Incubation
- None
Consumables
- HBO 100W/2 Mercury lamp bulb
La fluorescence est allumée mais je ne vois aucune lumière à l'échantillon
Ceci peut survenir lorsqu'un élément n'est pas dans la bonne configuration le long du trajet lumineux. Les schémas du trajet lumineux peuvent vous aider à identifier le problème.
- Vérifiez que l'alimentation de la lampe au mercure est allumé (#2)
- Vérifiez que la lampe au mercure située en haut à l'arrière du microscope est allumée. Elle dégage une forte chaleur. Attention aux risques de brulure.
- Vérifiez que les filtres de densité neutre (N16, N4, N2) situés en arrière sur le coté droit du microscope sont en position basse (out)
- Vérifiez que l'obturateur manuel situé en arrière sur le coté droit du microscope est en position basse (out)
- Vérifiez que les diaphragmes d'ouverture (A) et de champ (F) situés en arrière sur le coté droit du microscope sont ouverts en position haute
- Vérifiez que la tourelle de cube est sur une position non vide (1: I3 ou 2: N2.1)
Puis-je utiliser ce microscope pour observer des cellules en culture?
- Non. C'est un microscope droit conçu pour l'observation de spécimens montés entre lame et lamelles (d'épaisseur 0.17mm).
- Aucune étiquette