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Olympus BX63 upright microscope

P-G Desmarais Building, Room 5308-2
Advanced Microscope Tier 1 usage price

Instrument awarded to Dr Marina Martinez by the Canadian Foundation for Innovation (CFI)

  • Applications

    • Transmitted light, Bright-field
    • Polarized light, DIC

    • Fluorescence

    • Color camera

  • Light sources

    • LED lamp for transmitted light

    • X-Cite NOVEM-S XT910 for fluorescence

SourceExcitation wavelengths (nm)Compatible fluorophoresNominal power (mW)
365

DAPI, Hoechst


435
CFP
475
FITC, GFP
500-600-
[500-600]

509/22
[498-520]		YFP
554/23
[542-565]		TRITC
578/21
[567-588]		mCherry
635


Cy5


735


Cy7


  • Objectives

    1. 4x/0.16 Air WD 16

    2. 10x/0.4 Air WD 3.1

    3. 20x/0.75 Air WD 0.6

    4. 40x/0.95 Air WD 0.18

    5. 60x/1.35 Oil WD 0.15

    6. 100x/1.4 Oil WD 0.13

    7. Empty

PositionNomMarqueNom completIdentifiantOuverture numériqueImmersionDistance de travail (mm)Transmittance
(% [nm])		TechniqueÉpaisseur du couvre-objet (mm)
14x/0.16 AirOlympus4x/0.16 Air UPlanSApo

UPLXAPO4X.pdf

0.16Air16

>90% at 550nm

BF, Fluo-
210x/0.4 AirOlympus10x/0.4 Air
UPlanSApo		UPLXAPO10X.pdf0.4Air3.1

~90% at 550nm

BF, Pol, DIC, Fluo0.17
3

20x/0.75

Olympus20x/0.75 Air
UPlanSApo

UPLXAPO20X.pdf

0.75

Air

0.6

>90% at 550nm

BF, Pol, DIC, Fluo0.17
4

40x/0.95 Air

Olympus40x/0.95 Air
UPlanSApo

UPLXAPO40X.pdf

0.95

Air

0.18

~90% at 550nm

BF, Pol, DIC, FluoAdjustable 0.11-0.23
560x/1.35 OilOlympus60x/1.35 Oil 
UPlanSApo

UPLXAPO60XO.pdf

1.35
Oil
0.15

~90% at 550nm

BF, Pol, DIC, Fluo0.17
6

100x/1.4 Oil

Olympus100x/1.4 Oil 
UPlanSApo

UPLXAPO100XO.pdf

1.4
Oil
0.13

~90% at 550nm

BF, Pol, DIC, Fluo0.17
7

Vide









BF: Bright-field
Pol: Polarized light
DIC: Differential interference contrast
Fluo: Fluorescence

  • Filters

    1. Empty
    2. DAPI
    3. FITC
    4. TRITC
    5. mCherry
    6. Empty
    7. Empty
    8. U-FDICT DIC Analyzer (Nomarski prism)
PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
1Empty





2DAPIOlympusU-FUNA360-370 410LP420-460 

U-FUNA

3FITCOlympusU-FBNA470-495 505LP510-550 

U-FBNA

4TRITCOlympusU-FGWA530-550 570LP575-625 

U-FGWA

5mCherryOlympusU-FYW540-585 595LP600LP

U-FYW

6Empty





7Empty





8

DIC Analyzer

OlympusU-FDICT DIC---DIC Analyzer

  • Detector

    • Olympus DP80 Dual sensors (color and monochrome) CCD camera, 6.45 x 6.45 um pixel size,15 images/s at full frame 55% QE at 550nm
      Color mode 4080 x 3072 pixels, 24-bit
      Monochrome mode 1360 x 1024 pixels, 14-bit
      Olympus_DP80 Camera_User Guide.pdf

  1. Remove the dust cover from the microscope
  2. Turn on the computer (#1)
  3. Turn on the microscope power bar (#2)

  4. Turn on the microscope touchscreen (switch is located at the back on the right of the touch screen) (#3)

  5. Use your UdeM credentials to log in to Windows

    The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions in the First use section below.

  6. Start the software CellSens Dimension

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

This process will delete all experiment protocols and reset the software to the original settings for this specific microscope.

  1. If open, close the software CellSens Dimension and wait until it is completely closed (up to 30 seconds)
  2. On your Desktop open the folder Documentation
  3. Double-click Settings for BX-63
  4. A script will run and a black window will briefly appear
  5. You can now reopen the software CellSens Dimension
  • Files can be saved temporarily (during acquisition) to local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

Important

In any case, do not store your files on the C: drive.

  1. Save your data
  2. Close the software CellSens Dimension
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  4. Turn off the computer
  5. If oil objectives were used, clean it with lens cleaner and lens paper (not Kimwipes)
  6. Turn off the microscope power bar (#2)
  7. Replace the dust cover on the microscope

Important Reminders

  • Collect your samples, especially those in the microscope
  • Leave the microscope and workspace clean

The following schematics depict the light path for transmitted (bright-field and Phase Contrast) and reflected (fluorescence) lights.



  • Check filter matchings
  • Create script for cell sens personalization
  • Create BF light path
  • Improve installation setup
  • Buy multi-bandpass filters
  • System relocated to Room 5308-2
  • Added to Faces
  • XCite NOVEM-S Installed

Stand

  • Olympus BX63 Serial

Condenser

  • Motorized condenser

Stage

  • Motorized stage SHe Serial 127808

Workstation

  • HP Z4 Workstation
  • Intel Xeon W-2102 @ 2.9GHz
  • RAM 32 GB DDR4 2400 MHz
  • OS 1 TB GB SSD 550 MB/s
  • 4 TB HD Data Storage (2 x 2 TB spanned volume) 162 MB/s
  • Video Card nVidia Quadro P620 2GB DDR5 dedicated memory
  • Monitor Dell P2415Q display 24' 3840 x 2160 
  • Software CellSens Dimension v3.2

Consumables


Troubleshooting

  • Please ensure that the microscope touchscreen is turned on. The switch is not easily accessible and is located on the back at the right of the touchscreen pad.

FAQ

  • No. This is an upright microscope designed to look at specimen mounted between a slide and a coverslip

Core Facilities CIB               Structural Biology    |    Cytometry    |    Microscopy    |    Histology

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