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Nikon Eclipse E-600 upright microscope

Roger Gaudry Building, Room N-620

  • Applications

    • Bright-field

    • Phase Contrast

    • Polarized light

    • Fluorescence

    • Color camera

  • Light sources

    • Halogen lamp for 30 W transmitted light

    • Mercury lamp 100 W (350~600nm) for fluorescence 

Peak emission (nm)Power (mW)
3347
36545
40534
43643
54637
57926

HBO Mercury lamp emission spectra (Source)
Comparison HBO Mercury vs XCite Metal Halide Lamps (Source)
OSRAM HBO 100W/2 Mercury lamp manual (pdf)

  • Objectives

    • 10x/0.3 Air Ph1 WD 16

    • 40x/0.75 Air Ph2 WD 0.75

    • 100x/1.3 Oil Ph3 WD 0.2

PositionNameBrandFull nameProduct IDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCover glass thickness (mm)
110x/0.3 AirNikon10x/0.3 Air Plan Fluor Ph1 DLL
10x0.3AirPlan Fluor16>75% [400-800]
Max % @500nm		BF, Pol, PhC, Fluo0.17
2

40x/0.75 Air

Nikon40x/0.75 Air Plan Fluor Ph2 DLL
40x

0.75

Air

Plan Fluor0.72>80% [400-750]
Max % @500nm
BF, Pol, PhC, Fluo0.17
3

100x/1.3 Oil

Nikon100x/1.3 Oil Plan Fluor Ph3 DLL

100x

1.3
Oil
Plan Fluor0.2>75% [400-800]
Max % @500nm
BF, Pol, PhC, Fluo0.17
4Empty










5Empty










6Empty










  • Filter cubes

    • DAPI/Hoechst/AMCA

    • FITC/EGFP

    • TRITC/Rhodamine

    • TexasRed

PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
1DAPI/Hoechst/AMCAChromaCube set 31000v2350/50x
[325-375]		400LP460/50m
[435-485]
2FITC/EGFPChromaCube set 41001

480/40x
[420-500]

505LP

535/50m
[510-560]
3TRITC/RhodamineChroma

Cube set 41002c

545/30x
[530-560]		570LP620/60m
[590-650]
4Texas RedChroma

Cube set 41004

560/55x
[532-588]

595LP645/75m
[607-682]
5Empty





6Empty





  • Detector

    • Color CMOS Nikon DS-Ri2 4908 x 3264 pixels,14-bit, 6 images/s at full frame

  1. Turn on the computer (#1)
  2. Turn on the microscope power bar (#2)

  3. If fluorescence is required, turn on the mercury lamp power supply unit (#3A) and press ignition (#3B)

    Mercury lamp must be on for at least 30 min before being turned off and vice-versa

  4. Log in Windows using your UdM credentials
  5. Start-up NIS-Elements

The first time you log in the computer, you need to import the microscope settings into the software. To do this follow the instructions Software setup.

  1. Save your data
  2. Close NIS-Elements
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  4. If used, clean the oil objective with lens cleaner and lens paper
  5. If fluorescence was used, turn off the mercury lamp power supply unit (#3A)
  6. Turn off the microscope power bar (#2)
  7. Wait until the lamps have cooled and cover the microscope

Important Reminders

  • Take back your samples including ones in the microscope
  • Leave the microscope and the working area clean
  • Mercury lamp must be on for at least 30 min before being turned off and vice-versa
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

In any case, your files should be removed from the C: drive.


This process is required the first time you are using the instrument. You will usually do it during the training session. It can also be performed if something is not working properly or if you want to reset the software interface.

This process will delete all experiment protocols and restore the original parameters for the microscope.

  1. Close NIS-Elements
  2. Wait until the complete closure of NIS-Elements
  3. Open the folder Desktop\Logiciels
  4. Open the software NIS Settings Utility
  5. Click on the Import tab
  6. Click on Browse
  7. Navigate to your desktop
  8. Select the file Nikon-E600 Settings.bin
  9. Click Select
  10. Select all items
  11. Click Import
  12. Click OK
  13. Close the NIS Settings
  14. Open NIS-Elements


The following schematics depict the light path for transmitted (bright-field, Phase Contrast, Polarized light) and reflected (fluorescence) lights.

Nikon E600 Lightpath (pdf)

  • Adjust focus drive jitter
  • Add a holder for polarized light analyzer
  • Nikon Preventive Maintenance
  • Adjustment and cleaning Stage Nosepiece, condenser, filter turret, focus drive, shutters
  • 100x slightly damaged but not the lens
  • Fluorescence cubes damaged: FITC TexasRed
  • Focus drive has a jitter
  • Replacement mercury lamp bulb HBO 1003W/2
  • 256 GB SSD added in workstation for OS
  • Windows 10 Installation
  • BIOS updated to v2.47
  • Camera Firmware updated to v2.11
  • NIS-Elements Basic Research v4.6 64-bits installed
  • Creation NIS Elements Settings
  • FITC and TxRed cubes excitation and emission filters slightly damaged. Dichroic OK

Stand

  • Nikon Eclipse E600 upright Serial 725540

Light sources

  • Transmitted light Halogen 12V 100W Serial 01875599
  • Reflected light
    • Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
    • Bulb housing model LH M100C-1 Serial 039326

Condenser

  • Condenser Universal C-CU Serial 081901
  • Lens Dry NA 0.9
  • Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty

Objectives

  • 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
  • 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
  • 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
  • Empty
  • Empty
  • Empty

Stage

  • Manual Stage TBD

Filters

  • DAPI/Hoechst/AMCA

  • FITC/EGFP

  • TRITC/Rhodamine

  • TexasRed

Detector

  • Color Camera Nikon DS-Ri2 Serial 701234

Workstation

  • HP Z440 Workstation
  • Intel Xeon E5-1630 v3 @ 3.7GHz
  • RAM 32 GB DDR4 2133 MHz  (4 x 8 GB)
  • OS 256 GB SSD 550 MBs
  • 2 TB HD Data Storage 150 MBs
  • Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
  • Monitor HP Z24i display 24' 1920 x 1200

Consumables


Troubleshooting

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

  • Turn off NIS-Elements
  • Turn on the camera (both the microscope power bar and the camera need to be ON
    (the camera is usually ON, the switch on the top of the camera)
  • Check the camera is correctly connected to the computer via a USB cable
  • Turn on NIS-Elements


FAQ

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.


Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)

Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)


Core Facilities CIB               Structural Biology    |    Cytometry    |    Microscopy    |    Histology

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