• Zen 2.6 updated hotfix 12
• Microsoft Windows update
• Laser output measured in spinning disk mode at the sample with 10x objective, 1x Extended tubelens, 100% laser power and ALL lasers ON:
  • 405nm: 1.08 mW StdDev 0.01mW

  • 488nm: 3.3 mW  StdDev <0.01mW (3.7 mW if laser 405 is OFF)

    405nm and 488nm Interactions

    When both 405nm and 488nm laser are ON simultaneously, the output power is decreased by 11%.

  • 561nm: 2.6 mW StdDev <0.01mW
  • 639nm: 1.37 mW StDev 0.02 mW

• Control for vibrations during construction work on the 4th floor

• Error when the definite focus is used (unexpected error the definite focus did not respond)
• To solve it:
  • Turn off the Zen Software
  • Turn off the microscope power bar (#3)
  • Turn off the definite focus by pressing and holding the definite focus button
  • Turn on the definite focus by pressing once the definite focus button
  • Wait until the definite focus display shows "Detecting stand"
  • Turn on the microscope power bar (#3)
• Confirm that the definite focus is showing in the microscope tactile display
• Open Zen software and run an experiment using the definite focus to confirm it is fully functional

• Added to wiki

Can I use this microscope to look at cell in a dish?

• Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
• The objectives are optimized to image through thin glass bottom multi-well plates
• You may also image specimen mounted between a slide and a 0.17mm thick coverslip