GE InCell Analyzer 6000 High content microscope

JA Bombardier Building, Room 3129
Instrument awarded to Dr. Steve Michnick by the Canadian Foundation for Innovation (CFI)

Applications
- Transmitted light
- Pseudo phase contrast and DIC
- Fluorescence
- High-throughput imaging
- Long-term imaging
Light sources
- LED for transmitted light
- Toptica iChrome MLE for fluorescence

Source	Polychroic	Excitation wavelength (nm)	Compatible fluorophores	Nominal Power (mW)
405nm	390/40	[370-410]	DAPI, Hoechst	50
488nm	482/18	[473-491]	FITC, GFP, YFP	25
561nm	564/9	[559-569]	Cy3, DsRed, TxRed	30
642nm	640/14	[632-647]	Cy5, Cy5.5	50

Objectives
1. 10x/0.45 Air WD 4.0
2. 20x/0.75 Air WD 1.0
3. 40x/0.6 Air WD 2.7-3.7
4. 60x/0.95 Air WD 0.15

Position	Name	Brand	Full name	Identifier	Workinf distance (mm)	Transmittance (% [nm])	Technic	Coverslip thickness (mm)
1	10x/0.45 Air	Nikon	10x/0.45 Air Plan Apo M25x0.75	MRD00101	4.0	>80% [TBD-TBD]	BF, p-PhC, p-DIC, Fluo	0.17
2	20x/0.75 Air	Nikon	20x/0.75 Air Plan Apo DIC N2 M25x0.75	MRD00201	1.0	>80% [TBD-TBD]	BF, p-PhC, p-DIC, Fluo	0.17
3	40x/0.6 Air	Nikon	40x/0.6 Air Plan Fluor ELWD M25x0.75	MRH08430	2.7-3.7	>80% [TBD-TBD]	BF, p-PhC, p-DIC, Fluo	0.17
4	60x/0.95 Air	Nikon	60x/0.95 Air Plan Apo M25x0.75	MRD00600	0.15	>80% [TBD-TBD]	BF, p-PhC, p-DIC, Fluo	0.17

BF: Bright-field
p-PhC: Pseudo-phase contrast
p-DIC: Pseudo-Differetial interference contrast

Can I use this microscope to look at cell in a dish?

No. This a microscope designed to acquire images of specimen in multi-well plates
You may also image specimen mounted between a slide and a 0.17mm thick coverslip

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