During this procedure, you will: Once completed, your sample will be ready for acquisition. | UI Expand |
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| title | Mise au point initialeInitial Focus |
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| - If this has not been done yet, select the lowest-magnification objective, 4×.
| Info |
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The 4× objective is the safest to use due to its long working distance (17 mm). The sample will appear in sharp focus well before the objective gets close. It is recommended to always focus first using the safest objective. Since the objectives are parafocal, focusing with these objectives will make it easier to locate the sample when switching to higher-magnification objectives. |
- Place the test slide on the microscope stage, with the coverslip toward the objective.
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Using a test slide will significantly reduce the time needed to set up the instrument. |
- If necessary, move the stage so that the sample is centered under the objective
- Select the desired imaging mode (Brightfield or Fluorescence)
- Ensure that the light is clearly visible through the eyepieces before continuing
- Adjust the focus using the main focus knob while looking through the eyepieces until the image is perfectly sharp
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| title | Mise au point secondaireSecondary focus |
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| | Warning |
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First perform the initial focusing with the safest objective before selecting a higher-magnification objective. |
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| title | Focusing with air objectives |
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| After completing the initial focus: - Select the desired air objective (10×, 20×, 40×, or 60×).
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The 60× objective is the best air objective because it has the highest numerical aperture (0.85). |
- Adjust the focus using the fine focus knob while looking through the eyepieces until the image is perfectly sharp.
Your sample is now ready for acquisition! |
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