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Nikon Ei Microscope

Desmarais Building, Room 5308
Simple Microscope usage price
This Instrument was  intrusted to the CI2B by the department of pathology

 

Applications

  • Upright microscope
  • Widefield imaging
    • Brightfield
  • Color camera

Light sources

  • LED lamp for transmitted light

Objectives

  1. 4x/0.10 Air

  2. 10x/0.25 Air

  3. 20x/0.4 Air

  4. 40x/0.65 Air

PositionNameBrandFull nameIDMagnificationNumerical ApertureImmersionTypeWorking distance (mm)Transmittance
(% [nm])		TechniqueCover glass thickness (mm)
14x/0.1
Air		Nikon4x/0.1 Air
BE2 Plan		MRN700424x0.1AirAchromat25

>90%

BF0 - 0.17
210x/0.25
Air		Nikon10x/0.3 Air
BE2 Plan		MRN7010210x0.25AirAchromat6.7

>80%

BF0 - 0.17
3

20x/0.4
Air

Nikon20x/0.4 Air
BE2 Plan		MRN7020220x0.4

Air

Achromat3.7>80%BF0.17
4

40x/0.65
Air

Nikon40x/0.65 Air
BE2 Plan

MRN70402

40x

0.65

Air

Achromat0.6>90%BF0.17

Detector

  • Nikon Digital Sight 1000

CameraNikon Digital Sight 1000

Sensor Type

CMOS

Sensor Category

Color

Nb Pixels

 2 M

Pixel Layout

1920 x 1080

Pixel size

7.3 um

Sensor size

5.57 mm x 3.13 mm 

Sensor diameter

 mm

Bit depth

12-bit
Speed at full resolution30 images/s

Max QE

- %
Readout noisee⁻

Cooling

Passive

Dark Current

- e⁻/pixel/sec

Full well capacity

e-

Dynamic Range

1: -

Interface

USB 2.0

Mount

C-mount

Nikon_Camera_Digital Sight_Brochure.pdf 

  1. If not already done, turn on the computer (#1) and log in to Windows using your UdeM credentials
  2. Remove the dust cover from the microscope
  3. Turn on the camera (#2)

  4. Turn on the LED lamp on the front of the microscope (#3)

  5. Start-up NIS-Elements

During this procedure, you will:

  • Set up the microscope in a safe configuration

  • Load your sample

  • Find and adjust the focus

Once completed, your sample will be ready for acquisition.

  • If this has not been done yet, select the lowest-magnification objective, 4×.

The 4× objective is the safest to use due to its long working distance (17 mm). The sample will appear in sharp focus well before the objective gets close. It is recommended to always focus first using the safest objective. Since the objectives are parafocal, focusing with these objectives will make it easier to locate the sample when switching to higher-magnification objectives.

  • Place the test slide on the microscope stage, with the coverslip toward the objective.

Using a test slide will significantly reduce the time needed to set up the instrument.

  • If necessary, move the stage so that the sample is centered under the objective
  • Select the desired imaging mode (Brightfield or Fluorescence)
  • Ensure that the light is clearly visible through the eyepieces before continuing
  • Adjust the focus using the main focus knob while looking through the eyepieces until the image is perfectly sharp

First perform the initial focusing with the safest objective before selecting a higher-magnification objective.

After completing the initial focus:

  • Select the desired air objective (10×, 20×, 40×, or 60×).

The 60× objective is the best air objective because it has the highest numerical aperture (0.85).

  • Adjust the focus using the fine focus knob while looking through the eyepieces until the image is perfectly sharp.

Your sample is now ready for acquisition!

  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
  • Save your data
  • Close NIS-Elements
  • Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  • Select the 4x objective and place the objectives in a safe position
  • Turn off the microscope (#3)
  • Turn off the camera (#2)
  • Turn off the computer
  • Cover the instrument with the protective dust cover
  • Take back your samples including ones in the microscope
  • Leave the microscope and the working area clean

Added to the wiki

Stand

Nikon Eclipse Ei upright Serial 077218

Light sources

  • LED light

Condenser

  • Manual Condenser 
  • Lens Dry NA

Objectives

  1. 4x/0.10 Air MRN70042

  2. 10x/0.25 Air MRN70102

  3. 20x/0.4 Air MRN70202

  4. 40x/0.65 Air MRN70402

Stage

  • Manual Stage

Detector

  • Nikon Digital Sight 1000 Serial 210250
  • 0.55x relay lens

Workstation

  • HP ProDesk 600 G1 SFF Workstation Serial
  • I155434-CIB
  • Intel Core i5-4590 @ 3.3 GHz
  • Motherboard HP 18E7 Chipset Intel Q85 
  • RAM 6 GB DDR3 800 MHz (4 + 1 + 1 GB)
  • OS 500 GB SSD 500 MB/s
  • Video Intel Graphic HD 4600
  • Monitor LG E2240 display 22' 1920 x 1080
  • Software NIS-Elements F v5.42.1

Anti-vibration table

  • None

Consumables

  • None

Manuals

Troubleshooting

This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.

  • Turn off NIS-Elements
  • Turn on the camera (both the microscope power bar and the camera need to be ON
    (the camera is usually ON, the switch is on the top of the camera)
  • Check the camera is correctly connected to the computer via a USB cable
  • Turn on NIS-Elements

FAQ

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.

Core Facilities - CI2B               Structural Biology    |    Cytometry    |    Microscopy    |    Histology    |    Instruments & Services
Content published under CC BY-SA 4.0. Sharing allowed under the same license with attribution: "Original content by l'Institut Courtois d'innovation biomédicale, used under CC BY-SA 4.0"