- Created by Nicolas Stifani, last modified by André Julien Lévesque on Feb 09, 2024
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Zeiss Axio-Imager Z2 fully motorized upright microscope
J-A Bombardier Building, Room 3223-01
Advanced Microscope Tier 1 usage price
See on the
Training and booking available upon request to the corresponding platform manager
- Consult the and of the
- Objective 10-0.3 Plan NeoFlura #420340-9901-000 added
- Parafocality adjustment using 100x-1.4 as reference
- Condenser removed and cleaned (broken glass)
- Zen 2.6 updated hotfix 12
- Microsoft Windows updated
- X-Cite Liquid light guide replaced (Transmittance was 75% remaining)
- Fluorescence Light bulb replaced (was 2593 hours)
- Power output at the sample using 20x objective GFP cube 488nm is 116mW
- Objective parafocality adjusted
- Objective Focus speed was changed 10x: 7; 20x: 6; 4-x: 5; 63x: 3; 100x-1.3: 3; 100x-1.4: 3.
- Condenser lens cleaned (oil)
- Data storage OK
- Added to wiki
Stand
- Zeiss AxioImager Z2 upright Serial: 35230001000
Part Number: 430000-9902
System ID 1022265893 Camera adapter Model 60N-C, 1", 1x, Model: 426114
Motorized Neutral density filters for transmitted light
Manual Field diaphragm for transmitted light
- Manual polarizer
- Left imaging port with manual splitter camera adapter Model 60N-C, 1", 1x, Model: 426114
- Trinocular with 100% ocular 40% occular/70% camera and 100% manual splitter
- 3mm liquid light guide #805-0038
- Zeiss 423302-0000 Collimator
- Motorized Aperture diaphragm
- Motorized Fluorescence field diaphragm
Light sources
- Transmitted Halogen light 12V 100W HAL 100 #423000
- TBD Filters
- X-Cite exacte 200 W Model XCT10A Serial: XCT10A-0156
Condenser
- Motorized condenser #424201-9902
Lens NA 0.9 WD TBD Part Number: TBD
- Manual polarizer
Filter turret 6 positions manual
H Empty
- D Darkfield
- DIC III #426706
- Ph3
- DICII #426702
Ph 2
- DIC 426701
- Ph 1
Objectives
10x/0.30 Air WD 5.30 DIC I Plan-NeoFluar M27 420340-9901-000
20x/0.80 Air WD 0.61 DIC II Plan-Apochromat W0.8x1/36" 440640-9903-000
- 40x/0.75 Air WD 0.71 DIC II EC Plan-NeoFluar M27 420360-9900-000
63x/1.40 Oil WD 0.19 DIC III Plan-Apochromat M27 420782-9900-000
- 100x/1.30 Oil WD 0.20 DIC III Plan-NeoFluar M27 420490-9900-000
100x/1.40 Oil WD 0.17 DIC III Plan-Apochromat M27 420792-9900-000
Stage
- Motorized stage Zeiss AIM System #2502000124
- Remote control joystick
- Inserts
- Slide only
Filters
10-positions motorized filter wheel #424913 #424905-0160-810
- DAPI Zeiss Filter Set 49 cube 424933
- GFP Zeiss Filter Set 38 cube 424933
- YFP Zeiss Filter Set 46 cube 424933
- DsRed Zeiss Filter Set 43 cube 424933
- TxRed Zeiss Filter Set 45 cube 424933
- Cy3.0 Chroma #SP102v1 C162410
- Cy3.5 Chroma #SP103v1 C162411
- Cy5 Chroma #49009 C162412
- DIC Analyzer Zeiss 424932-01
- Empty
Detector
- Photometrics Prime Camera sCMOS 2048 x 2048 pixels, 16-bit, 30 images/s at full resolution, detector size 13.312mm x 13.312mm (18.8 mm diagonal). Serial: A18B202001. Cameralink cable and PCIe adapter available for imaging 100 images/s
Workstation
- HP Z800 Workstation Serial: CZC1473Y0Q Part number: WJ112ECJ#AK6
- 2 x Intel Xeon X5650 2.66 GHz
- RAM 24 GB DDR3 1333 MHz ECC (12 x 2 GB)
- OS 500 GB SSD 410 MB/s
- 2 TB HD Data Storage (2 x 1 TB spanned volume) 110 MB/s
- Video Card ATI FirePro V5800 1 GB DDR5
- Monitor Dell ST2410 24' 1920 x 1080
- Software Zen Blue 2.6 Hotfix 12
Incubation
- None
Consumables
- 3mm liquid light guide X-Cite #805-0038
- X-Cite exacte 200 W replacement bulb X-Cite Lumen Dynamics Part number 012-66000R
- 12V 100W halogen lamp OSRAM XenoPhot #64623 HLX
Can I use this microscope to look at cell in a dish?
- No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.
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