- Created by Nicolas Stifani, last modified on Jul 30, 2021
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HP Z840 Deconvolution and Image Analysis Workstation
Roger Gaudry R-619
Applications
2D and 3D Deconvolution
Image processing
Image Analysis
Quantification
Processor
2 x Intel Xeon E5-2623 v4 2.60 GHz
RAM
128 GB (8x16GB) ECC DDR4 2400 MHz
Drives
- 512 GB NVMe at 2500 MB/s OS
- 4 TB (2x2TB) at 170 MB/s spanned volume for storage
Video Card
NVidia GTX 1080 with 8GB DDR5 dedicated memory
Monitor
HP Z24n 24" IPS display native resolution 1920 x 1200
- OS
- Windows 10 21H1
- Softwares
ImageJ
FIJI
Microsoft Office 2016
NIS-Elements AR v5.02 64-bits
NIS Elements Offline Deconvolution v4.11 64-bits
- Turn on the computer
- Log in Windows using your UdM credentials
- Start-up NIS-Elements Offline Deconvolution
- Save your data
- Close NIS-Elements
- Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
- Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
- At the end of each session, copy your data to your external drive and delete it from the local C: drive
- You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).
In any case, your files should be removed from the C: drive.
- Bring a network cable to connect directly to Ti2 microscope workstation
- Windows 10 UdeM Image installation
- Change the 2nd network adapter configuration with fix IP to enable for computer to computer communication
- Change sharing policies of public document folder
Stand
- Nikon Eclipse E600 upright Serial 725540
Light sources
- Transmitted light Halogen 12V 100W Serial 01875599
- Reflected light
- Power supply Nikon Mercury lamp Type C SHG1 Serial D12139
- Bulb housing model LH M100C-1 Serial 039326
Condenser
- Condenser Universal C-CU Serial 081901
- Lens Dry NA 0.9
- Filters: Empty, Ph1, Ph2, Ph3, DICM, DICH, Empty
Objectives
- 10x/0.3 Plan Fluor Ph1 DLL WD 16 Air
- 40x/0.75 Plan Fluor Ph2 DLL WD 0.72 Air with PF40 Wollaston prism
- 100x/1.3 Plan Fluor Ph3 DLL WD 0.2 Oil with PF/PA 100 Oil Wollaston prism
- Empty
- Empty
- Empty
Position | Name | Brand | ID | Magnification | Numerical Aperture | Immersion | Type | Working distance (mm) | Transmittance (% [nm]) | Technique | Cover glass thickness (mm) |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | 10x/0.3 Air | Nikon | 10x/0.3 Air Plan Fluor Ph1 DLL | 10x | 0.3 | Air | Plan Fluor | 16 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 |
2 | 40x/0.75 Air | Nikon | 40x/0.75 Air Plan Fluor Ph2 DLL | 40x | 0.75 | Air | Plan Fluor | 0.72 | >80% [400-750] | BF, Pol, PhC, Fluo | 0.17 |
3 | 100x/1.3 Oil | Nikon | 100x/1.3 Oil Plan Fluor Ph3 DLL | 100x | 1.3 | Oil | Plan Fluor | 0.2 | >75% [400-800] | BF, Pol, PhC, Fluo | 0.17 |
4 | Empty | ||||||||||
5 | Empty | ||||||||||
6 | Empty |
Filters
DAPI/Hoechst/AMCA
FITC/EGFP
TRITC/Rhodamine
TexasRed
Position | Cube name | Brand | ID | Excitation Filter | Dichroic mirror | Emission Filter |
---|---|---|---|---|---|---|
1 | DAPI/Hoechst/AMCA | Chroma | Cube set 31000v2 | 350/50x [325-375] | 400LP | 460/50m [435-485] |
2 | FITC/EGFP | Chroma | Cube set 41001 | 480/40x [420-500] | 535/50m [510-560] | |
3 | TRITC/Rhodamine | Chroma | 545/30x [530-560] | 570LP | 620/60m [590-650] | |
4 | Texas Red | Chroma | 560/55x [532-588] | 595LP | 645/75m [607-682] | |
5 | Empty | |||||
6 | Empty |
Detector
- Color Camera Nikon DS-Ri2 Serial 701234
Workstation
- HP Z440 Workstation
- Intel Xeon E5-1630 v3 @ 3.7GHz
- RAM 32 GB DDR4 2133 MHz (4 x 8 GB)
- OS 256GB SSD 550 MBs
- 2TB HD Data Storage 150 MBs
- Video Card NVIDIA Quadro K620 2 GB DDR3 dedicated memory
- Monitor HP Z24i display 24' 1920x1200
Consumables
- Mercury lamp bulb OSRAM HBO 1003W/2 100W
- Tungsten Halogen Lamp OSRAM 64623 HLX 100W 12V
NIS-Elements shows an error: Camera Driver...
This happens when NIS-Elements does not find the camera. It is usually because the camera is not powered on.
- Turn off NIS-Elements
- Turn on the camera (the microscope power bar and the camera switch on the top of the camera)
- Check the USB conexion between the camera and the computer
- Turn on NIS-Elements
Can I use this microscope to look at cell in a dish?
- No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.
Cells courtesy of Benoit Bessette and Monique Vasseur (Biochemistry)
Images courtesy of Dr Shirley Campbell and Emilie Fiola-Masson (Pharmacology)