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Zeiss Axio-Imager Z2 fully motorized upright microscope

Roger Gaudry B-301


  • Objective 10-0.3 Plan NeoFlura #420340-9901-000 added
  • Parafocality adjustment using 100x-1.4 as reference
  • Condenser removed and cleaned (broken glass)
  • Zen 2.6 updated hotfix 12
  • Microsoft Windows updated
  • X-Cite Liquid light guide replaced (Transmittance was 75% remaining)
  • Fluorescence Light bulb replaced (was 2593 hours)
  • Power output at the sample using 20x objective GFP cube 488nm is 116mW
  • Objective parafocality adjusted
  • Objective Focus speed was changed 10x: 7; 20x: 6; 4-x: 5; 63x: 3; 100x-1.3: 3; 100x-1.4: 3.
  • Condenser lens cleaned (oil)
  • Data storage OK
  • Added to wiki

Stand

  • Zeiss AxioImager Z2 upright Serial: 35230001000
    Part Number: 430000-9902
    System ID 1022265893

  • Camera adapter Model 60N-C, 1", 1x, Model: 426114

  • Motorized Neutral density filters for transmitted light

  • Manual Field diaphragm for transmitted light

  • Manual polarizer
  • Left imaging port with manual splitter camera adapter Model 60N-C, 1", 1x, Model: 426114
  • Trinocular with 100% ocular 40% occular/70% camera and 100% manual splitter
  • 3mm liquid light guide #805-0038
  • Zeiss 423302-0000 Collimator
  • Motorized Aperture diaphragm
  • Motorized Fluorescence field diaphragm

Light sources

  • Transmitted Halogen light 12V 100W HAL 100 #423000
    • TBD Filters
  • X-Cite exacte 200 W Model XCT10A Serial: XCT10A-0156

Condenser

  • Motorized condenser #424201-9902

  • Lens NA 0.9 WD TBD Part Number: TBD

  • Manual polarizer

  • Filter turret 6 positions manual

    1. H Empty

    2. D Darkfield
    3. DIC III #426706
    4. Ph3
    5. DICII #426702

    6. Ph 2

    7. DIC 426701
    8. Ph 1

Objectives

  1. 10x/0.30 Air WD 5.30 DIC I  Plan-NeoFluar M27 420340-9901-000

  2. 20x/0.80 Air WD 0.61 DIC II Plan-Apochromat W0.8x1/36" 440640-9903-000

  3. 40x/0.75 Air WD 0.71 DIC II EC Plan-NeoFluar M27 420360-9900-000

  4. 63x/1.40 Oil WD 0.19 DIC III Plan-Apochromat M27 420782-9900-000

  5. 100x/1.30 Oil WD 0.20 DIC III Plan-NeoFluar M27 420490-9900-000

  6. 100x/1.40 Oil WD 0.17 DIC III Plan-Apochromat M27 420792-9900-000 

Stage

  • Motorized stage Zeiss AIM System #2502000124
  • Remote control joystick
  • Inserts
    • Slide only

Filters

10-positions motorized filter wheel #424913 #424905-0160-810

  1. DAPI Zeiss Filter Set 49 cube 424933
  2. GFP Zeiss  Filter Set 38 cube 424933
  3. YFP Zeiss  Filter Set 46 cube 424933
  4. DsRed Zeiss  Filter Set 43 cube 424933
  5. TxRed Zeiss  Filter Set 45 cube 424933
  6. Cy3.0 Chroma #SP102v1 C162410
  7. Cy3.5 Chroma #SP103v1 C162411
  8. Cy5 Chroma #49009 C162412
  9. DIC Analyzer Zeiss 424932-01
  10. Empty

Detector

  • Photometrics Prime Camera sCMOS 2048 x 2048 pixels, 16-bit, 30 images/s at full resolution, detector size 13.312mm x 13.312mm (18.8 mm diagonal). Serial: A18B202001. Cameralink cable and PCIe adapter available for imaging 100 images/s

Workstation

  • HP Z800 Workstation Serial: CZC1473Y0Q Part number: WJ112ECJ#AK6
  • 2 x Intel Xeon X5650 2.66 GHz
  • RAM 24 GB DDR3 1333 MHz ECC (12 x 2 GB)
  • OS 500 GB SSD 410 MB/s
  • 2 TB HD Data Storage (2 x 1 TB spanned volume) 110 MB/s
  • Video Card ATI FirePro V5800 1 GB DDR5
  • Monitor Dell ST2410  24' 1920 x 1080
  • Software Zen Blue 2.6 Hotfix 12

Incubation

  • None

Consumables


Can I use this microscope to look at cell in a dish?

  • No. This is an upright microscope designed to look at specimen between a glass slide and a 0.17mm thick cover slip.


Core Facilities CIB               Structural Biology    |    Cytometry    |    Microscopy    |    Histology

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