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Nikon TE2000-E inverted microscope

Bombardier Building, Room 3132
Instrument awarded to Dr Steve Michnick by the Canadian Foundation for Innovation (CFI)
Advanced Microscope Tier 1 usage price

  • Applications

    • Transmitted light, Bright-field

    • Fluorescence

    • Live imaging
    • Time-lapse imaging

  • Light sources

    • Halogen lamp for transmitted light

    • X-Cite exacte

  • Objectives

    1. 4x/0.1 Air
    2. 10x/0.30 Air Ph1
    3. 20x/0.45 Air Ph1
    4. 40x/0.95 Air
    5. 60x/1.4 Oil Ph3
    6. 100x/1.45 Oil
PositionNameBrandFull nameIdentifierWorking distance (mm)Transmittance
(% [nm])		TechniquesCover glass thickness (mm)
14x/0.10 AirNikon4x/0.1 Plan

MRL00042

30>80% [400-900]BF, Fluo-
210x/0.30 Air Ph1Nikon10x/0.30 Plan Fluor Ph1 DL

MRH20101
MRH20105

15.2>80% [440-700]BF, PhC, Fluo1.2 !
320x/0.45 Air Ph1Nikon20x/0.45 Plan Fluor ELWD Ph1 ADL

MRH48230
MRH48220

7.4>80% [350-870]BF, PhC, Fluo0-2
440x/0.95 AirNikon40x/0.95 Plan Apo DIC M/N2

MRD00400
MRD70470

0.14>80% [440-760]BF, Fluo0.11-0.23
560x/1.4 Oil Ph3Nikon60x/1.4 Plan Apo
Ph3 DM

MRD31600

0.21 BF, PhC, Fluo0.17
6100x/1.45 OilNikon100x/1.45 Plan Apo Lambda  OFN25 DIC N2

MRD01905
MRD71970

0.13>80% [460-720]BF, Fluo0.17

  • Filters

    1. Dichroic DAPI\FITC\TRITC

    2. Dichroic CFP\YFP\Cy5

    3. Dichroic CFP\YFP\mCherry

    4. DAPI
    5. Cy3n
    6. Cy5.5
  • Also available

    • CFP

    • FITC

    • YFP

    • Cy3.5

    • mCherry

    • Cy5

Filter cubes

PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
1Dichroic
DAPI\FITC\TRITC		Chroma86013 v2
86013bs
To be used in combination with excitation and emission filters
2

Dichroic
CFP\YFP\Cy5

Chroma86008 v2


86008bs


To be used in combination with excitation and emission filters
3

Dichroic
CFP\YFP\mCherry

Chroma

89006 ET


69008bs
To be used in combination with excitation and emission filters
4DAPIChroma

31000 v2

AT350/50x

400dclp

D460/50m


5Cy3nChroma

SP102 v1

HQ546/11xQ557lp

HQ567/15m


6Cy5.5Chroma

41022

HQ665/45x

Q695lpHQ725/50m

 Excitation filters

PositionNameBrandIDExcitation FilterMatching dichroic mirrorMatching Emission FilterComments
1DAPIChroma86013 v2 Exc 1AT350/50x

86013bs

 		S457/17m
2

CFP

Chroma86008 v2 Exc 1

S430/25x

86008bs
69008bs

S465/30m
3FITCChroma86013 v2 Exc 2

S484/15x

86013bs

S517/30m


4

YFP

Chroma86008 v2 Exc 2

S510/20x

86008bs
69008bs

S550/50m


5TRITCChroma86013 v2 Exc 3S555/25x

86013bs

S605/40m


6

mCherry

Chroma89006 ET Exc 3

ET572/35x

69008bs

ET632/60m


7

Cy5

Chroma86008 v2 Exc 3

S622/36x

86008bs

S700/75m
8

Empty







Emission filters

PositionNameBrandIDMatching Excitation FilterMatching dichroic mirrorEmission FilterComments
1DAPIChroma86013 v2 Em 1AT350/50x

86013bs

 		S457/17m
2

CFP

Chroma86008 v2 Em 1

S430/25x

86008bs
69008bs

S465/30m
3FITCChroma86013 v2 Em 2

S484/15x

86013bs

S517/30m


4

YFP

SemrockFF01-550/49-26

S510/20x

86008bs
69008bs

F01-550/49-25


5TRITCChroma86013 v2 Em 3S555/25x

86013bs

S605/40m


6

mCherry

SemrockFF01-641/75-25

ET572/35x

69008bs

FF01-641/75-25


7

Cy5

Chroma86008 v2 Em 3

S622/36x

86008bs

S700/75m
8

Empty







9

Shutter







10

Shutter







 Also available

PositionNameBrandIDExcitation FilterDichroic mirrorEmission FilterComments
-eCFPChroma

49001 ET

ET436/20x

T455lp

ET480/40m


-cGFPChroma

31044 v2

D436/20x

455dclp

D480/40m


-FITCChroma

41001

HQ480/40x

Q505lp

HQ535/50m


-YFPChroma

49003 ET

ET500/20x

T515lp

ET535/30m


-Cy3.5Chroma

SP103 v1

HQ581/10x

Q593lp

HQ617/40m


-mCherryChroma

49008 ET

ET560/40x

T585lpxr

ET630/75m


-Cy5Chroma

49006 ET

ET620/60x

T660lpxr

ET700/75m


  • Detector

    • Hamamatsu ORCA Flash v2 CMOS Monochrome Camera 2048x2048 pixels, 16-bit, 30 images/s at full frame (100 images/s at full frame with camera link connector) Serial 002162

  1. Turn on the computer (#1)
  2. Remove the cover from the microscope
  3. Turn on the X-Cite exacte light (#2)
  4. Turn on the microscope power bar (#3)
  5. Use your UdeM credentials to log in to Windows
  6. The first time you use the instrument, you need to import the microscope settings into the software. To do this follow the instructions First use protocol.
  7. Start the software NIS
  1. Save your data
  2. If the oil objective was used, clean it with lens cleaner and lens paper (not Kimwipes)
  3. Select the lowest magnification objective
  4. Close the software NIS
  5. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  6. Turn off the computer
  7. Turn off the X-Cite exacte light (#2)
  8. Turn off the microscope power bar (#3)
  9. Cover the microscope

Important Reminders

  • Collect your samples, especially those in the microscope
  • Leave the microscope and workspace clean
  • Files can be saved temporarily (during acquisition) to local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create one folder per laboratory using the principal investigator's last name. Inside, create a folder per user using the following nomenclature (First Name_Last Name).

Important

In any case, do not store your files on the C: drive.

When using the microscope for the first time, you need to import the microscope settings into the software. You will usually do this during the training session.
This procedure can also be performed if something is not working properly and if you want to reset the software to its original settings.

This process will delete all experiment protocols and reset the software to the original settings for this specific microscope.

  1. If open, close the software NIS and wait until it is completely closed (up to 30 seconds)
  2. On your Desktop open the Softwares folder
  3. Open NIS Settings Utility
  4. Click on the Import tab
  5. Click on Browse
  6. Navigate to your Documents
  7. Select the file NIS Settings for Nikon TE2000E.bin
  8. Click Select
  9. Select all items
  10. Click Import
  11. Click OK
  12. Close NIS Settings Utility
  13. You can now reopen NIS
Available manuals
  • Fix camera adapter
  • Complete installation
  • Complete cleaning

Stand

  • Nikon TE2000-E inverted
  • Nikon T-HUBC Serial 750110
  • Nikon PFS T-PFS 810060
  • Nikon T-RCP Serial 750104

Light sources

  • Transmitted Halogen light Nikon TE2-PS100W Serial 505320 12V
    • GIF Filter
    • Diffusion
    • Infra-red
    • Neutral color
    • Transmitted light shutter uniblitz VCM D1 Serial A607295
  • X-Cite exacte Model XCT10A 200 W Seria;l XCT10A-0337
    • EXFO Collimator 810-00030
    • Prior Shutter HF201HT Serial 7332
    • Prior 8 position motorized excitation filter wheel 61817
    • Nikon Manual Neutral density filters ND8 ND4
    • Manual Fluorescence field diaphragm
    • Manual Fluorescence shutter

Condenser

  • Motorized condenser

  • Lens LWD NA 0.52

  • Filter turret 5 motorized positions

    1. A BF

    2. PhL
    3. Ph1
    4. Ph2
    5. Ph3

Objectives

  • See description

Stage

  • Prior Proscan 2 H30V4 Serial 62040
  • Remote control joystick Prior CS152v2 Serial R0772128
  •  Inserts
    • 1 slide
    • Multi-well plate
    • Adapter for 3cm dish

Filters

  • See description

Detector

  • Hamamtsu ORCA Flash V2 CMOS Model C11440-22CU Serial 002162 V2
  • Monochrome Camera 2048 x 2048 pixels
  • Pixel  6.5 um x 6.5 um
  • Force air cooled at -10C
  • Effective sensor area 13.312 mm (H) × 13.312 mm (V
  • Dark curent 0.06 electron/pixel/s
  • Reading noise 1.6 electrons (r.m.s) at standard scan
  • Dynamic range 1 : 18 000
  • Spectral response >40% between 400-850nm. Max 80% at 550nm
  • Hamamatsu_Orca Flash 4.0_V2_C11440-22CU_Instruction Manual.pdf

Workstation

  • HP Z440 Workstation
  • Intel Xeon E5-1620 v4 @ 3.5GHz
  • RAM 32 GB DDR4 2400 MHz ECC (4 x 8 GB)
  • OS 500 GB SSD 530 MB/s
  • 4 TB HD Data Storage (2 x 2 TB spanned volume) 130 MB/s
  • Video Card nVidia GTX 1080 8GB DDR5 dedicated memory
  • Monitor HP Z24i display 24' 1920x1200
  • Software NIS-Elements AR v5.02

Consumables

  • Liquid Light Guide


Troubleshooting

This can happen when the acquisition software is turned on before the camera has fully initialized.

  • Turn off NIS-Elements
  • Ensure the Initialize light at the back of the camera no longer blinks
  • Start NIS-Elements

FAQ

  • Yes. This is an inverted microscope designed to look at specimen in a dish or a multi-well plate
  • The objectives are optimized to image through thin glass bottom multi-well plates
  • You may also image specimen mounted between a slide and a 0.17mm thick coverslip
  • For long timelapse, be aware of photo-toxicity



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Content published under CC BY-SA 4.0. Sharing allowed under the same license with attribution: "Original content by l'Institut Courtois d'innovation biomédicale, used under CC BY-SA 4.0"

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