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Leica DM2000 Upright Microscope

Desmarais Building, Room 3248
Available upon request to Dr Samaha Lab
Instrument awarded to Dr Samaha

 

Applications

  • Upright microscope

  • Widefield imaging

    • Brightfield

    • Fluorescence
  • Color camera



Description

Light sources

  • Halogen lamp 30 W for transmitted light

  • Mercury lamp 100 W (350~600 nm) for fluorescence

Peak emission (nm)Nominal power (mW)
3347
36545
40534
43643
54637
57926

Objectives

  1. 4x/0.13 Air

  2. 10x/0.3 Air

  3. 20x/0.5 Air

  4. 40x/0.75 Air

Filters

  1. I3 (GFP, YFP)
  2. N2.1 (TRITC)
  3. -
  4. -
  5. L5 (GFP) 480/40 505 527/30
PositionNameBrandIDExcitationDichroicEmissionComments
1I3LeicaI3470/40
[450-490]		510LP515LP
[520+]		Emission filter is very broad.
Be aware of bleed-through.
2N2.1LeicaN2.1

537/45
[515-560]

580LP

590LP
[595+]		Emission filter is very broad.
Be aware of bleed-through.
3-






4-






5L5





Detector

  • Leica DFC425C
CameraLeica DFC425C

Sensor Type

CCD

Sensor Category

Color

Nb Pixels

 5 M

Pixel Layout

2592 x 1944

Pixel size

2.78 µm × 2.78 µm 

Sensor size

8.10 mm × 6.64 mm

Sensor diagonal

 8.93 mm

Bit depth

14-bit
Speed at full resolution3 fps

Max QE

?
Readout noise10e-

Cooling

0C Pelletier

Dark Current

?

Full well capacity

 ?

Dynamic Range

800:1

Interface

Firewire

Mount

c-Mount

  1. If not already done, turn on the computer (#1) and log in to Windows using your UdeM credentials
  2. Remove the dust cover from the microscope
  3. If fluorescence is required, turn on the mercury lamp power supply unit (#2)

    Mercury lamp must be on for at least 30 min before being turned off and vice-versa

  4. If transmitted light is required, turn on the halogen lamp on the right side of the microscope (#3)
  5. Start LAS 
  6. When using the software for the first time, you will be asked for registration and activation. 
    1. Select Do not tell me about this again at the bottom left
    2. Click Close
  • Files can be saved temporarily (during acquisition) on the local C: drive (desktop)
  • At the end of each session, copy your data to your external drive and delete it from the local C: drive
  • You can store your files on the D: drive (Data Storage). If you do, please create a folder per laboratory using the principal investigator last name. Within, create one folder per user (Firstname_Lastname).

In any case, your files should be removed from the C: drive.

  1. Save your data
  2. Close LAS
  3. Transfer your data to the D: drive (Data Storage) or to your external drive and delete it from the local C: drive
  4. Select the lowest magnification objective and place the objectives in a safe position
  5. If transmitted light was used, turn off the halogen lamp on the right side of the microscope (#3)
  6. If fluorescence was used and the mercury lamp has been ON for at least 30 minutes, turn off the power supply unit (#2)

  7. Turn off the computer

  8. Wait until the lamps have cooled and cover the instrument with the protective dust cover

Important Reminders

  • Take back your samples including ones in the microscope
  • Leave the microscope and the working area clean

Log

Test and Migrate windows 11

  • Test slides made and brought to the microscope
  • Added to Wiki
  • Computer replacement
  • Windows 10 installation
  • Network connection
  • Mercury lamp alignment

Technical Datasheet

Stand

  • Leica DM2000 upright microscope Serial 3312222-022011
  • Leica Trinocular 11551511 BZ00
  • Camera Adapter Leica 11541544

Light sources

  • Transmitted Halogen 12 V 30 W P/N 11038101180000
  • Reflected light

    • Power supply Ebq 100-04-L Serial 11 1111 0103B00931

    • Leica Mercury lamp housing Product ID 11504114

Condenser

  • Leica 11501183 BZ:02

  • Condenser filters

    • DF Filters 501158 (looks like phase ring)

Objectives

  1. Nikon Plan Fluor 4x/0.13 MRH000041 (should not be used)

  2. Leica HCX PL Fluotar 10x/0.3 506505

  3. Leica HCX PL Fluotar 20x/0.5 506503

  4. Leica HCX PL Fluotar 40x/0.75 506144

  5. Empty
  6. Empty

Stage

  • Manual Stage #11888186

Filters

  1. Leica I3
  2. Leica N2.1
  3. Empty
  4. Empty
  5. Empty

Cube Leica 11513882

Detector

  • Leica DFC425C Serial 535111411 Color Camera 2592 × 1944 pixels, 14-bit monochrome, 36-bit color mode, 6 image/s at full resolution

Workstation

  • Asus P6X58D-E Workstation
  • I155435-CIB
  • Motherboard Intel X58
  • BIOS 0803 2012-08-06
  • Processor Intel Intel Core i7-950 @ 3.0 GHz
  • RAM 12 GB DDR3 1333 MHz (2 x 1 GB + 3 x 2 GB + 1 x 4 GB)
  • Drives
    • OS 500 GB SSD 382 MB/s
    • Data Storage 500 GB HD 85 MB/s
  • Video Card AMD Radeon HD 5670 1 GB DDR5 1.2 TFLOPS
  • Monitor Viewsonic VX922 19' 1280 x 1024
  • OS Windows 11 23H2
  • Software LAS v4.9

Incubation

  •  None

Consumables

Troubleshooting

This can happen when something is not setup properly along the light path.

  1. Ensure the mercury lamp power supply (#2) is turned ON
  2. Ensure the mercury lamp located at the back of the microscope is shining. It should generate some heat. Beware of burn risks
  3. Ensure the neutral density filters (N16, N4, N2) located at the back on the right side of the microscope are out (low position)
  4. Ensure the manual shutter located at the back on the right side of the microscope is open (low position)
  5. Ensure the aperture (A) and field (F) diaphragms located at the back on the right side of the microscope are opened (high position)
  6. Ensure the filter cube is on a non-empty position (1: I3 ou 2: N2.1)

FAQ

No. This is an upright microscope designed to observe specimen mounted between a slide and a 0.17 mm thick coverslip.

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